VAF Funded Abstracts

The VAF has provided many thousands of dollars in grants supporting research to advance ocular health in animals. Nearly every grant has resulted in presentations of the funded research projects at the American College of Veterinary Ophthalmologists and other conferences. Abstracts of these presentations are published each year in the Veterinary Ophthalmology journal.



Comparison of cleaning and sterilization protocols of diamond burr tips used in Diamond Burr Debridement (DBD) for canine superficial chronic corneal epithelial defects. KC Banks, EA Giuliano, DE Stalla, FE Bunyak, TA White, LO Schultz.

Comparison of two different visual aid devices in affecting navigation in a population of irreversibly blind dogs. KM Bedard, KE Myrna, KA Diehl.

Adaptation of the melan‐100 for video capture chromatic pupillometry to evaluate dogs with sudden acquired retinal degeneration syndrome. AE Cooper, DJ Maggs, S Kim, A Marangakis, T Nguyen, MJ Motta, SM Thomasy.

Pentacam® HR evaluation of surgically induced corneal astigmatism in canines undergoing bilateral phacoemulsification. SL Pederson, AM Cleymaet, AM Hess, KL Wotman, KS Freeman.

Detection of viral DNA in canine lobular orbital adenomas. EA Schaefer, S Chu, KM Wylie, TN Wylie, JN Bryan, JW Pearce, BK Flesner.

Evaluation of the major histocompatibility complex (MHC) class II as a candidate gene for sudden acquired retinal degeneration syndrome in dachshunds. SJ Stromberg, SM Thomasy, AD Marangakis, S Kim, AE Cooper, DL Bannasch.

Development and validation of new methods to visualize conventional aqueous outflow pathways in canine glaucoma. MR Telle, KC Snyder, K Oikawa, LBC Teixeira, JA Kiland, E Bentley, A Huang, and GJ McLellan.



Detection of papillomavirus DNA in canine lobular orbital adenoma. EA Schaefer, S Chu, JN Bryan, JW Pearce and BK Flesner.

Safety and efficacy of treatment with mycophenolate mofetil in dogs with sudden acquired retinal degeneration syndrome. WM Young, A Oh, J Williams, ML Foster and FM Mowat.

Identification of novel lymphatic channels within the canine anterior UVEA using LYVE-1 and CD31. A Dubin, EJ Ehrhart and KS Freeman.

Effects of the heat shock protein inhibitor, 17AAG, on corneal stromal haze using an in vivo rabbit phototherapeutic keratectomy model. SG Edwards, PH Kass, M Ali, AD Marangakis, VK Raghunathan, CJ Murphy, and SM Thomasy (original grant to Sydney Cartiff).

Effect of bone marrow-derived autologous mesenchymal stem cells and stem cell supernatant on equine corneal wound healing in vitro. AB Sherman, LV Schnabel and BC Gilger.



The effect of trypan blue on posterior capsule opacification in an ex vivo canine model. BM Brash, DA Wilkie, AJ Gemensky-Metzler and HL Chandler.

Therapeutic potential of the anti-fibrotic drug suberanilohydroxamic acid (SAHA) in dogs. KM Gronkiewicz, EA Giuliano, K Kuroki, F Bunyak, and RR Mohan.

Development of a novel ex vivo equine corneal model. Todd L Marlo, Elizabeth A Giuliano, Ajay Sharma, and Rajiv R Mohan.

Effect of topical ophthalmic latanoprost 0.005% solution alone and in combination with diclofenac 0.1% solution in normal horses. KL Tofflemire, RD Whitley, G Ben-Shlomo, RA Allbaugh, AN Griggs, TS Strong, and EM Whitley.



Characterization of an immune response following intravitreal delivery of an adeno-associated viral vector. RF Boyd, SL Boye, TJ Conlon, KE Erger, J Sun, SE Boye, WW Hauswirth, AM Komaromy, SM Petersen-Jones, and JT Bartoe.

Tear film concentrations of doxycycline following oral administration in normal dogs. SP Collins, AL Labelle, L Dirikolu Z Li MA Mitchell, and RE Hamor.

Ocular and systemic safety of topical suberoylanilide hydroxamic acid (SAHA) in dogs. KM Gronkiewicz, EA Giuliano, A Sharma, and RR Mohan.

Species variation in mucin expression from corneal epithelium. BC Leonard, B Yanez-Soto, VK Raghunathan, NL Abbott, and CJ Murphy.

Histologic characteristics of feline diffuse iris melanomas. KT Wiggans, CM Reilly, and DJ Maggs.



Decorin-pei nanoconstruct attenuates equine corneal fibroblast differentiation. KS Donnelly, EA Giuliano, A Sharma, A Tandon and RR Mohan.

Determination of uv-a/riboflavin corneal collagen crosslinking (CXL) penetration depth in ex vivo rabbit and equine corneas using fluorescent biomarkers. NS Gallhoefer, BM Spiess, F Guscetti, M Hilbe, S Hartnack, F Hafezi and SA Pot.

Effects of grape seed extract, lutein, and omega-3 fattyacids on lens epithelial cell behavior in vitro and ex vivo. EJ Miller, AJ Gemensky-Metzler, DA Wilkie, CMH Colitz and HL Chandler.

Active, latent and total TGF- b2 concentrations in the aqueous humor of dogs with open angle glaucoma. EM Scott, AM Komaromy, CD Harman, K Koehl, CE Plummer and GJ McLellan.

In vivo ocular imaging of the canine anterior segment using optical coherence tomography and confocal microscopy. AR Strøm, DE Cortes, CA Rasmussen, SM Thomasy, K McIntyre, Lee S, PH Kass, MJ Mannis and CJ Murphy.

Ultrasound biomicroscopy compared to standard ocular ultrasonography for detection of canine uveal cysts. LN Taylor, WM Townsend, HG Heng, J Stiles and GE Moore.



Canine corneal fibroblast and myofibroblast transduction with aav5. AP Bosiack, EA Giuliano, R Gupta and RR Mohan.

Clinical and histopathologic effects of diode laser endoscopic cyclophotocoagulation in the normal equine eye. JT Harrington, RJ McMullen Jr, JM Cullen, NB Campbell and BC Gilger.

Impact of modified cyclosporine a on lens epithelial cell and corneal endothelial viability. EA Lutz, DA Wilkie, AJ Gemensky-Metzler and HL Chandler.



Efficacy and safety of mitomycin C as an agent to treat corneal scarring in horses using an in vitro model. DG Buss, A Sharma, EA Giuliano and RR Mohan.

Assessment of antiviral efficacy against feline herpesvirus and cytotoxicity of famciclovir and its metabolites, and intracellular accumulation of penciclovir. AD Groth, KQ Nguyen, HK Kado-Fong, MT Contreras, SM Thomasy and DJ Maggs.

The effects of hyaluronic acid on lenticular epithelial migration in vitro.  DJ Haeussler Jr, EA Lutz, DA Wilkie, AJ Gemensky-Metzler and HL Chandler.

Effects of cyclophosphamide immunosuppression and myelosuppression in adult dogs with latent canine herpesvirus-1 infection. P Mundy, EC Silva and EC Ledbetter.

Immunohistochemical and immunopathologic characterization of superficial stromal immune mediated keratitis in horses. DO Pate.



Cellular localization of Visudyne as a function of time after local injection for photodynamic therapy: an investigation into the mechanisms of tumor cell death. L. D. Barnes, E. A. Giuliano and J. Ota.

Expression of matrix metalloproteinases in feline eyes with uveitis and lens luxation. J. J. Broadwater, C. M. H. Colitz, R. R. Dubielzig, S. M. Carastro and H. L. Chandler.

Topical ocular corticosteroid administration in dogs with experimentally induced latent canine herpesvirus-1 infection. N. C. Kice, E. C. Ledbetter, S. G. Kim, R. B. Matusow and E. J. Dubovi.

Local anesthetic retrobulbar block reduces postoperative pain following enucleation of canine eyes: a randomized, placebo controlled, double-masked clinical trial. K. E. Myrna, E. Bentley and L. J. Smith.

The effect of intravitreal ciliary neurotrophic factor (CNTF) on structure, function and gene expression of normal canine retinas. A. T. Parton-Corr, G. D. Aguirre, W. A. Beltran and A. M. Komaromy.

Pharmacokinetics of famciclovir and penciclovir following single-dose oral administration of famciclovir to cats. S.M. Thomasy, T. Whittem, S. D. Stanley and D. J. Maggs.

Tear, cornea, and aqueous humor concentrations of ciprofloxacin and moxifloxacin after topical application in normal horses. H. D. Westermeyer, D. V. H. Hendrix, D. A. Ward and S. R. Cox.

Canine preiridal fibrovascular membranes: morphologic and immunohistochemical investigations. M. K. Zarfoss, C. B. Breaux, H. E. Whiteley, R. E. Hamor, J. A. Flaws, P. Labelle and R. R. Dubielzig.



Transcleral delivery of antiglaucoma drugs: pharmacokinetics and pharmacodynamics in normotensive rabbits. T. M. Fife, C. Mendes, G. C. Matsutani, P. M. Evans, G. G. Gum, R. H. Scagliotti and R. A. P. Carvalho.

In vitro and in vivo evaluation of an equine intraocular lens, R. J. McMullen Jr, J. H. Salmon, M. G. Davidson and B. C. Gilger.

Selenium functionalized intraocular lenses inhibit posterior capsule opacification in an ex vivo canine lens capsular bag assay. S. A. Pot, H. L. Chandler, C. M. H. Colitz, E. Bentley, R. R. Dubielzig, T. S. Mosley, T. W. Reid and C. J. Murphy.

Oxidative stress in glaucomatous retinas of dogs. D. S. Zirofsky, J. E. Madl and J. R. Gionfriddo.



The effect of photodynamic therapy on squamous cell carcinoma in a murine model: an evaluation of time between intralesional injection to laser irradiation. L. D. Barnes, E. A. Giuliano, J. Ota, L. A. Cohn and C. P. Moore.

The effect of a single dose of topical 0.005% latanoprost and 2% dorzolamide/0.5% timolol combination on the blood aqueous barrier in normal dogs. N. S. Johnstone McLean and D. A. Ward.

Combined effect of topical flurbiprofen and latanoprost on normal canine eyes: a controlled study. C. G. Pirie, L. Maranda and S. Pizzirani.

Identification of ocular matrix metalloproteinases present within the aqueous humor and iridocorneal drainage angle tissue of normal and glaucomatous canine eyes. William L. Weinstein, Ursula M. Dietrich, John S. Sapienza, K. Paige Carmichael, Phillip A. Moore and Thomas M. Krunkosky.



Expression of cyclin dependent kinase inhibitor P15INK4bin canine refractory corneal ulcers. M. J. Hodgson, H. L. Chandler, W. W. Miller and C. M. Colitz.

Pathogenic phenotype and genotype of canine ocular Pseudomonas aeruginosa isolates. E. C. Ledbetter, T. J. Kern, R. C. Riis, N. L. Irby, J. M. Scarlett, F. W. Wallace-Gadsden and S. M. J. Fleiszig.

Microvessel obliteration in the retinas of dogs with primary glaucoma. B. G. Mangan, J. R. Gionfriddo, R. R. Dubielzig and J. E. Madl.

Local photodynamic therapy for squamous cell carcinoma in a murine model. J. Ota, E. A. Giuliano, L. A. Cohn, M. R. Lewis and C. P. Moore.

Identification of ocular matrix metalloproteinases (MMPs) within the aqueous humor of normal canine eyes and canine eyes with glaucoma. W.L. Weinstein, U. M. Dietrich, J. S. Sapienza, K. P. Carmichael, P. A. Moore and T. Krunkosky.



In vitro fungistatic and fungicidal activity of silver sulfadiazine and natamycin on pathogenic fungi isolated from equine eyes. C. M. Betbeze, C. C. Wu, J. Stiles, S. G. Krohne and M. J. Finn.

Aqueous humor concentrations of Voriconazole®after topical and oral administration in horses. A. B. Clode, J. Davis, J. Salmon, T. Miller Michau and B. C. Gilger.

Evaluation of the effect of laser cyclophotocoagulation on the success of glaucoma drainage implant surgery in a rabbit model. D. W. Esson, G. Gum, J. Brinkis, R. H. Scagliotti, M. B. Sherwood, E. M. Sampson and G. S. Schultz.

The effect of topical sodium chloride ophthalmic ointment on corneal thickness and healing in dogs with nonhealing or recurrent epithelial erosions. C. S. Fahrer, T. Blocker and R. H. Scagliotti.

Long-term histologic effect of diode laser transscleral cyclophotocoagulation (TSCP) on the normal equine eye. V. J. Kuonen, A. J. Gemensky-Metzler, D. A. Wilkie, C. M. H. Colitz and S. E. Weisbrode.

Quantification of FHV-1 DNA from the conjunctiva of cats with and without conjunctivitis. H. C. Low, C. C. Powell and M. R. Lappin.



Matrix metalloproteinases in precorneal tear film from cats with ulcerative keratitis. D. H. Agapito, M. B. Berman, T. Blocker, G. Kennard, R. E. Merideth, J. Urbanz, C. Warren,§ D. J. Maggs and L. Meade-Tollin.

Identification of lactoferrin in canine and feline tears. B. J. Holmberg, D. J. Maggs and B. L. Lönnerdal.

Treatment of equine periocular squamous cell carcinoma with photodynamic therapy: 5 cases. G. Klauss, E. A. Giuliano, D. L. McCaw, I. MacDonald, P. J. Johnson, L. E. Galle and C. P. Moore.